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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: J Photochem Photobiol B. 2009 Nov 26;98(1):77–94. doi: 10.1016/j.jphotobiol.2009.11.007

Figure 1.

Figure 1

Illustration showing different fluorescence imaging paths used in the scope of preclinical studies. High resolution, high sensitivity and high specificity images can be rendered down to sub-micron resolution in vitro to study cellular and sub-cellular molecular processes. The top-right part of the figure shows two-photon microscopy images of mouse hippocampal neuron and glial cells transfected with GFP and RFP, respectively (courtesy of Dr Paul De Koninck, www.greenspine.ca). Animal models can be used for ex vivo studies of tissue slices as well as for whole-body in vivo studies. Ex vivo slices shown (middle-right images) correspond to brain tissue with glioma cells highlighted with fluorescence from GFP and the endogenous molecule Protoporphyrin IX. The in vivo whole-body image (lower-right in the figure) corresponds to a fluorescence tomography image associated with PpIX contrast from a brain tumor model.