Table 2. Identification of a candidate novobiocin resistance mutation in gyrB.
| Nonsynonymous SNPs and corresponding amino acids in gyrB codonsd | ||||||||||||
| 137 | 144 | 165 | 220 | 246 | 299 | 336 | 381 | 567 | ||||
| Strain | Subspeciesa | STb | Novc | 411 | 431 | 493 | 658 | 736 | 896 | 1006 | 1141 | 1700 |
| DAR1263 | SHH | 1 | S | Asp | Arg | Ile | Glu | Ser | Ala | Ile | Ile | Ala |
| GAT | CGT | ATA | GAA | TCT | GCA | ATA | ATC | GCT | ||||
| DAR1286 | SHH | 10 | S | |||||||||
| ... | ... | ... | ... | ... | ... | ... | ... | ... | ||||
| DAR1932 | SHH | 13 | S | Pro | Val | Val | ||||||
| ... | ... | ... | ... | C.. | ... | G.. | G.. | ... | ||||
| DAR2030 | SHH | 1 | S | |||||||||
| ... | ... | ... | ... | ... | ... | ... | ... | ... | ||||
| DAR3114 | SHH | 17 | S | Pro | Val | Val | ||||||
| ... | ... | ... | ... | C.. | .T. | ... | ... | .T. | ||||
| DAR3383 | SHH | 26 | S | Val | Lys | Pro | Val | |||||
| ... | ... | G.. | A.. | C.. | ... | G.. | ... | ... | ||||
| DAR1684 | SHN | 2 | R | Leu | ||||||||
| ... | .T. | ... | ... | ... | ... | ... | ... | ... | ||||
| DAR1919 | SHH | 8 | R | Glu | Leu | Pro | ||||||
| ..G | .T. | ... | ... | C.. | ... | ... | ... | ... | ||||
| DAR3358 | SHN | 2 | R | Leu | ||||||||
| ... | .T. | ... | ... | ... | ... | ... | ... | ... | ||||
| DAR3374 | SHN | 16 | R | Leu | Pro | |||||||
| ... | .T. | ... | ... | C.. | ... | ... | ... | ... | ||||
| DAR3377 | SHN | 2 | R | Leu | ||||||||
| ... | .T. | ... | ... | ... | ... | ... | ... | ... | ||||
| DAR3384 | SHN | 2 | R | Leu | ||||||||
| ... | .T. | ... | ... | ... | ... | ... | ... | ... | ||||
a
SHN is S. hominis subsp. novobiosepticus, SHH is S. hominis subsp. Hominis.
b
ST is multilocus sequence type.
c
Nov is novobiocin susceptibility, S = sensitive, R = resistant.
d
In column header, top number is amino acid position, bottom number is nucleotide position; dots indicate identity with the sequence from strain DAR1263.