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. 2013 Jun 11;8(6):e66007. doi: 10.1371/journal.pone.0066007

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© 2013 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) RT-PCR analysis of virus accumulation in individual adult insects. An agarose gel electrophoresis showing virus free (VF; lane 1 and 2), RBSDV-infected (SI_RB; lane 3 and 4), RSV-infected (SI_RSV; lane 5 and 6) and doubly-infected (DI; lane 7 and 8) samples. Lengths of predicted PCR products: RBSDV S1, 691 bp; RSV RNA3, 936 bp. (B) Numbers of L. striatellus carrying single or double viruses or that were virus free from a total of 419 insects analyzed. (C) The proportions of L. striatellus of different genders or wing form [macropterous (long-winged) or brachypterous (short-winged)] in the insect populations carrying single or double viruses or that were virus free.