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. 2013 Jun 11;8(6):e66502. doi: 10.1371/journal.pone.0066502

Figure 1. ADIPOR1 is a direct target of miR-221/222.

Figure 1

(A) qRT-PCR analysis of MCF10A cells, 48 hours post transfection with either miR-Control or miR-221/222 mimic. Data represent means of triplicates ± SD. (B) ADIPOR1 mRNA expression data taken from microarray analysis of 32 luminal and 23 basal-like breast cancer cell lines. Data represented as boxplot, with whiskers indicating maximum and minimum values of the data set. (C) qRT-PCR analysis of ADIPOR1 mRNA expression versus miR-221 levels in 49 breast cancer cell lines. (D) qRT-PCR analysis of ADIPOR1 mRNA expression versus miR-221 levels in 27 primary breast tumors. (E) qRT-PCR analysis of ADIPOR1 mRNA expression versus miR-222 levels in 49 breast cancer cell lines. (F) qRT-PCR analysis of ADIPOR1 mRNA expression versus miR-222 levels in 27 primary breast tumors. (G) The predicted ADIPOR1 wild-type (WT) and mutant 3’UTR sequence for miR-221/222 is shown above. Below, MCF10A cells were co-transfected with the reporter constructs and an increasing titration of miR-221/222 mimic. Data represent means of triplicates ± SD.