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. 2013 Jun 11;8(6):e65940. doi: 10.1371/journal.pone.0065940

Figure 1. Hypoxia leads to upregulation of Sost gene expression.

Figure 1

(A) Increase of HIF-1α expression in RNA level in osteoblasts under hypoxia. RNA level was normalized to heat shock protein 90 (HSP90). A paired t-test was performed comparing control group (20% O2) and hypoxia group (1% O2). *: A star indicates statistical significance compared to control group. (B) Western blotting analysis of HIF-1α expression in protein level in osteoblasts under hypoxia. Heat shock protein 90 (HSP90) was used as a loading control. (C) Quantification of western blotting of HIF-1α and HSP90 expressions in protein levels. Protein level from normoxic condition (20%O2) group was normalized to a value of 1. (D) RNA expression level of Sost as determined by quantitative real-time RT-PCR. MC3T3 osteoblasts were cultured for 48 hr under hypoxia (1%O2). RNA was isolated and quantitated by real-time RT-PCR. The RNA level from normoxic condition (20%O2) group was normalized to a value of 1. Values were presented as the mean ±S.D. A paired t-test was performed comparing control group (20% O2) and hypoxia group (1% O2). *: A star indicates statistical significance compared to control group.