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. 2013 Jun 11;8(6):e65940. doi: 10.1371/journal.pone.0065940

Figure 4. Effect of HIF-1α on Sost promoter activity.

Figure 4

(A) HIF-1α activates the Sost promoter in a dose-dependent manner. HEK293 cells were transfected with a 1 kb Sost promoter-luciferase reporter gene without or with increasing amounts of an HIF-1α-expression plasmid as indicated. Luciferase activity was normalized by β-galactosidase activity. Values are presented as the mean ±S.D. (B) Jab1 does not activate Sost promoter activity. HEK293 cells were transfected with a 1 kb Sost promoter-luciferase reporter gene without or with increasing amounts of a Jab1-expression plasmid as indicated. Luciferase activity was normalized by β-galactosidase activity. Values are presented as the mean ±S.D.