Figure 6. IRAK-M^−/− BMDCs do not affect T_reg induction in vitro.

(A) BMDCs isolated from WT and IRAK-M^−/− mice were plated and pulsed with OVA for 2 hours before CD4+ T cells isolated from OT-II Foxp3-GFP animals were added to the wells in the presence of IL-2 and TGFβ for 72 hours. Cells were restimulated with PMA and ionomycin in the presence of monesin, and Foxp3-GFP expression in CD4⁺ T cells was measured by flow cytometry.Data are representative of three independent experiments. (B) Bar graph represents mean ± SD from data collected from three individual experiments performed in duplicate.