Figure 1. Expression pattern analysis of GhCAX3 in cotton responsive to various treatments.

Cotton seedlings were treated with 200 mM CaCl₂ (A), 400 mM NaCl (B), 100 µM ABA (C) and 15% (W/V) PEG (D). For cold stress measurement, seedlings were exposed to 4°C (E). Total RNA was isolated from leaf (L) tissues at indicated time points for GhCAX3 expression assay under Ca²⁺ treatment. Under salt, ABA and PEG treatment, total RNA from leaf (L) and root (R) tissues was isolated respectively at indicated time points. Expression analysis was tested by qRT-PCR. Grey and black column represent relative expression level of GhCAX3 with respect to UBQ7 under normal condition (CK) and various cues, respectively. (F) GhCAX3 transcripts in various tissues of cotton were detected by RT-PCR. Root, stem and leaf samples were collected from 3-week old seedlings, the other tissues were from mature cotton plants.