Figure 3. Sorafenib and Vorinostat interact to kill medulloblastoma and PNET cells. (A) VC312 cells were treated with vehicle or Sodium Valproate (1 mM). Thirty minutes later cells were treated with vehicle or sorafenib (1 μM; 3 μM; 6 μM). Cells were isolated 48 h later. Viability was determined by trypan blue exclusion (n = 3, ± SEM) *p < 0.05 greater than corresponding vehicle control treated cells. (B) DAOY cells were treated with vehicle or Sodium Valproate (1 mM). Thirty minutes later cells were treated with vehicle or sorafenib (2 μM; 4 μM; 6 μM). Cells were isolated 48 h later. Viability was determined by trypan blue exclusion (n = 3, ± SEM) *p < 0.05 greater than corresponding vehicle control treated cells. (C) D283 cells were treated with vehicle or Sodium Valproate (1 mM). Thirty minutes later cells were treated with vehicle or sorafenib (2 μM; 4 μM; 6 μM). Cells were isolated 48 h later. Viability was determined by trypan blue exclusion (n = 3, ± SEM) *p < 0.05 greater than corresponding vehicle control treated cells.