Figure 3. Intestinal qRT-PCR analysis of iron and copper homeostasis-related genes.

Expression of key genes (indicated in each panel) was determined by standard methods, with each experimental gene being normalized to expression of mouse cyclophilin mRNA (which did not vary significantly). Filled bars represent data from mice fed the control diet and open bars represent data from mice consuming the low-iron diet. Genotype is indicated below each bar. ^a,b,c,d Statistically different from one another within each panel (p<0.05). n = 4 for each genotype consuming the control diet and n = 6 for each genotype fed the low-iron diet. WT, wild-type. Bars (A–F) depict mean±SD.