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. 2013 Jun 11;8(6):e66370. doi: 10.1371/journal.pone.0066370

Table 1. Overview of the de novo assembly of the transcriptome of P. pruinosa.

Sequences control-callus salt-stressed callus desert-grown trees
Contig
Number of contigs 226 299 200 532 1 497 985
Length of all contigs (nt) 47 707 523 43 936 971 151 124 036
Average contig size 211 219 101
Range of contig length 75-3 357 75-3 970 75-2 331
N50 (bp) 1 259 274 90
Scaffolds
Number of scaffolds 119 471 106 812 161 908
Length of all scaffolds (nt) 45 468 086 41 913 454 36 670 030
Average scaffold sizes 381 392 226
Range of scaffold lengths 100-4 165 100-5 569 100-2 331
N50 (bp) 567 598 249
All Unigenes sequences
Number of Unigenes 122 883
Length of all Unigenes (nt) 64 580 690
Average Unigene size 526
Range of Unigene length 200-5 569
N50 (bp) 681
1

N50 size is a weighted median statistic indicating that 50% of the entire assembly resides in contigs/scaffolds of a length at least X.