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. 2013 Jun 11;8(6):e63686. doi: 10.1371/journal.pone.0063686

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© 2013 Zitomersky et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A Western blot of PSA from B. fragilis isolates grown in vitro after isolation from control, CD and UC (inflamed (I) a non-inflamed (NI) biopsies. Panel A: Lanes 1–6 contain B. fragilis isolates from the biopsies control subjects (4, 6, 13, 34, 68, and 1). Lanes 7–8 contain isolates from non-inflamed and inflamed biopsies from subject 23 with UC. Lanes 9–11 contain isolates from inflamed and uninflamed biopsies from subjects with UC: 32, 29 and 77. Panel B: Lane 1 contains B. fragilis type strain 9343 as a positive control. Lanes 2 and 3 contain isolates from a non-inflamed and then an inflamed biopsy from subject 47 with CD. Lanes 4–8 contain B. fragilis isolates from inflamed biopsies of subjects 26, 56, 64, 14, and 85 with CD.