Table 2. Primers used in this study.
Gene | Gene Product | Direction* | Oligonucleotide Sequence (5' à 3') | Reference |
adk | adenylate kinase | F | CATCATTCTTCTCGGTGCTC | This study |
R | AGTGCCGTCAAACTTCAGGTA | |||
gyrB | DNA gyrase subunit B | F | GTACGTTTCTGGCCTAGTGC | This study |
R | GGGTCTTTTTCCTGACAATC | |||
metE | methionine synthase | F | CGGGTGACTTTGCTTGGT | This study |
R | CAGATCGACTGGGCTGTG | |||
mdh | malate dehydrogenase | F | ATGAAAGTCGCTGTTATTGG | This study |
R | GCCGCTTGGCCCATAGAAAG | |||
R | TAGCTTGATAGGTTGGG | This study | ||
pntA | pyridine nucleotide transhydrogenase | F | CTTTGATGGAAAAACTCTCA | |
R | GATATTGCCGTCTTTTTCTT | This study | ||
F | GGCCAGCCCAAAATCCT | |||
purM | phosphoribosyl-formylglycinamide cyclo-ligase | F | GGTGTCGATATTGATGCAGG | This study |
R | GGAATGTTTTCCCAGAAGCC | |||
pyrC | Dihydroorotase | F | ATCATGCCTAACACGGTTCC | This study |
R | TTCAAACACTTCGGCATA | |||
ctxAB | cholera toxin | F | CTCAGACGGGATTTGTTAGGCACG | [69] |
R | TCTATCTCTGTAGCCCCTATTACG | |||
rtxA | repeat like toxin | F | GCGATTCTCAAAGAGATGC | [27] |
R | CACTCATTCCGATAACCAC | |||
sto/stn (NAG-ST) | heat-stable toxin | F | CCTATTCATTAGCATAATG | [12] |
R | CCAAAGCAAGCTGGATTGC | |||
tcp | toxin co-regulated pili | F1 | GTGACTGAAAGTCATCTCTTC | [44] |
R1 | AATCCGACACCTTGTTGGTA | |||
F2 | ATATGCAATTATTAAAACAGC | |||
R2 | TTATTATTACCCGTTGTCGG | |||
vcsC2 | inner membrane protein | F | GGAAAGATCTATGCGTCGACGTTACCGATGCTATGGGT | [12] |
R | CATATGGAATTCCCGGGATCCATGCTCTAGAAGTCGGTTGTTTCGGTAA | |||
vcsV2 | ATPase | F | ATGCAGATCTTTTGGCTCACTTGATGGG | [12] |
R | ATGCGTCGACGCCACATCATTGCTTGCT |