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. 2013 Jun 11;8(6):e65478. doi: 10.1371/journal.pone.0065478

Figure 6. The hippocampus of adult Nfix +/− mice remains misshapen.

Figure 6

No gross differences in the volume of hippocampal structures were detected between adult wild-type and Nfix +/− mice (A) using magnetic resonance imaging at a resolution of 30 µm (p>0.5). Haematoxylin staining of coronal brain sections (B and C) revealed that the CA1 pyramidal cell layer (arrowhead in C) and dentate gyrus (arrow in C) remained misshapen in adult Nfix+/− mice. Moreover the suprapyramidal blade of the dentate gyrus appeared thinner in adult Nfix +/− mice (C’) compared with wild-type mice (B’). B’ and C’ are magnified views of the boxed regions in B and C respectively. Scale bar (in C’): B, C 500 µm; B’, C’ 70 µm. Abbreviations: CA = Cornu ammonis; Py = pyramidal layer; Or = oriens layer; Rad = radiatum layer; MoDG = molecular layer of the dentate gyrus; GrDG = granule layer of the dentate gyrus; PoDG = polymorph layer of the dentate gyrus.