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. 2013 Jun 11;8(6):e65478. doi: 10.1371/journal.pone.0065478

Figure 7. Hippocampal neurogenesis is impaired in adult Nfix +/− mice.

Figure 7

Anti-PROX1 (A–B’), anti-GFAP (C–D’) and anti-DCX (E–F’) staining of coronal sections of adult wild-type and Nfix +/− brains. Immunostaining for PROX1 and subsequent quantification (A–B’) revealed that the thickness of the suprapyramidal blade of the dentate gyrus was reduced in adult Nfix +/− mice compared with wild-type mice (**p<0.01; G). There were also fewer GFAP-positive radial fibres (**p<0.01; H) and DCX-positive neurons (***p<0.001; I) per unit length of the dentate gyrus in adult Nfix +/− mice. A’, B’, C’, D’, E’ and F’ are magnified views of the boxed regions in A, B, C, D, E and F respectively. Scale bar (in F’) A, B, C, D, E, F 300 µm; A’, B’, C’, D’, E’, F’ 75 µm.