Table 1. Mean Fluorescent Intensity (MFI) of the 10 Leishmania isolates measured by Flow Cytometry.
Isolate Reference Code | Descriptive Code | Influx | efflux rate (slope “α”) | efflux rate (slope “β”) | |
“susceptible” Isolates | MCAN/CY/2005/CD45 | D2 | 4.87 | 0.16 | 0.16 |
MCAN/CY/2005/CD57 | D7 | 6.90 | 0.87 | 0.64 | |
MHOM/CY/2006/CH34 | H1 | 20.53 | 1.36 | 0.22 | |
MCAN/CY/2005/CD40 | D6 | 15.17 | 1.55 | 0.92 | |
MCAN/CY/2006/CD87 | D1 | 18.60 | 1.83 | 0.25 | |
“resistant” Isolates | MCAN/CY/2006/CD83 | D5 | 51.23 | 2.36 | 0.27 |
MCAN/CY/2005/CD40 | D8 | 37.27 | 2.88 | 0.68 | |
MCAN/CY/2006/CD85 | D4 | 25.37 | 3.53 | 0.97 | |
MCAN/CY/2006/CD80 | D3 | 60.25 | 4.26 | 2.48 | |
MHOM/CY/2006/CH33 | H2 | 30.83 | 6.00 | 0.50 |
D1-D8: Dog isolates, Leishmania infantum MON-1; H1-H2: Human isolates, Leishmania donovani MON-37; Influx, MFI on baseline; Slope “α”, rate of efflux of Rhod-123 in 120 minutes; Slope “β”, rate of efflux of Rhod-123 in 120 minutes in the presence of Verapamil hydrochloride.