Table 2. Proteins detected by proteomic analysis and Silver Nitrate Staining.

Title	Isolate code and spot number (SSP)	Mascot Score	MS Coverage	Protein MW	Method	Accession	pI-Value
Heat shock 70 kDa protein - L. donovani	H2/4719	108	27	71410	Tryptic Mass MALDI TOF	HSP70_LEIDO	5,2
Heat shock 70 kDa - related protein 1, mitochondrial precursor –L. infantum	D3/3719	207	47	68915	Tryptic Mass MALDI TOF	HSP71_LEIMA	5,4
Heat shock 70 kDa - related protein 1, mitochondrial precursor –L. infantum	D3/4716	154	35	68915	Tryptic Mass MALDI TOF	HSP71_LEIMA	5,4
Putative uncharacterized protein - L. infantum	D3/5218	135	56	36576	Tryptic Mass MALDI TOF	A4HXA8_LEIIN	5,6

Of the 1208 protein spots detected, 6 appeared to represent significantly different proteins between the two groups of parasites. Of those, 4 were detected only in the “resistant” isolates (H2, D3) and 2 in both groups but over-expressed in the “resistant” isolates (p<0.05).