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. 2013 Jun 11;8(6):e65750. doi: 10.1371/journal.pone.0065750

Figure 2. Purity of cultured LSCCs, sorting of SP and NSP cells, and purity of sorted SP and NSP cells.

Figure 2

(A) Purity of cultured LSCCs determined by flow cytometer using cytokeratin (CK) (pan) (98.32±0.93%) and (B) control. (C) Sorting of LSCCs using Hoechst 33342. The R2 gate represents the side population (SP) cells (4.45±1.07% of total cells) and the R4 gate is the non-SP (NSP) cells. (D) The SP proportion after verapamil treatment was 0.14±0.13%. The purity of the freshly sorted SP (99.25±0.23%) (E) and NSP cells (98.98±0.22%) (F).