Figure 4. Amino acid variability and frequency in A. marginale MSP1a tandem repeats.

The amino acid variability (A), comparison of the variability between tandem repeats at positions R1 and non-R1 (B) and frequency (C) were calculated per amino acid position in the MSP1a tandem repeats using the formula Variability = number of different amino acids at a given position/frequency of the most common amino acid at that position [50]. The one letter amino acid code was used to name the amino acids in (C) and the most frequent amino acids per position are colored in gray.