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. 2013 Jun 11;8(6):e65599. doi: 10.1371/journal.pone.0065599

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Mononuclear cells were isolated from the spleens, livers and lungs of wild-type (upper row) and Ly49A transgenic mice (lower row) and analyzed by flow cytometry. Software gates were set to identify NK cells (NK1.1+ CD3–), which were then examined for the expression of the maturation markers CD27 and CD11b.