Table 1. Estimates of oxidative stress and mutation frequency.
Line† | Relative ROS (SE)‡ | 8-oxodG (SE)§ | µBS ¶ | µG-TO-T |
523 | 471.4 (75.8)* | 54.92 (7.4)* | 3.163E-09 | 3.987E-05 |
526 | 269.2 (57.3) | 35.95 (4.6) | 2.446E-09 | 1.417E-05 |
529 | 282.8 (49.2) | 33.72 (1.1) | 1.845E-09 | 2.080E-05 |
553 | 261.5 (48.6) | 26.43 (7.8) | 2.890E-09 | 3.687E-05 |
574 | 350.4 (38.4)* | 50.79 (10.4)* | 1.757E-09 | 1.982E-05 |
MA mean | 328.2 (39.6) | 40.72 (5.4) | – | – |
N2 ancestor | 192.9 (29.6) | 23.97 (8.7) | – | – |
Baer mutation accumulation (MA) line number from the Baer et al. (2005) experiment [28].
Relative reactive oxygen species (ROS) levels expressed as means (standard error) of relative fluorescence units.
Indicates significantly different from N2 ancestor.
Means (standard error) of 8-oxo-7,8-dihydro-2′-deoxyguanosine, or 8-oxodG, are reported as ×109 damaged bases per nanogram of DNA.
See Materials and Methods for calculations of point estimates of the frequencies of base substitutions (µBS) and G-to-T transversions (µG-TO-T).