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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: Neurobiol Dis. 2011 Mar 21;43(1):142–151. doi: 10.1016/j.nbd.2011.03.002

Figure 3.

Figure 3

Integration Mapping of the Tg(SMN2)89Ahmb transgene. A) Genome walking was used to clone the integration site of Tg(SMN2)89Ahmb, which was shown to be within intron 4 of the mGluR7 gene. B) Presentation of the sequence at the integration site. Sequence with a white background is from the transgene while sequence in gray is from mGluR7. C) A 3-primer PCR assay of the integration site provides a genotyping assay capable of distinguishing between WT, hemizygous, and homozygous mice.