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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: Dev Biol. 2012 Mar 10;365(2):363–375. doi: 10.1016/j.ydbio.2012.03.004

Fig. 6.

Fig. 6

Gro/Grg4 and FoxD4/5 co-operate to cause transcriptional repression. The percentages of embryos showing decreased expression of zic1, zic3, or irx1 after injection of: 100 pg wt-FoxD4/5 mRNA (Fox-100), 10 pg wt-FoxD4/5 mRNA (Fox-10), 10 pg Gro/Grg4 mRNA (Grg4-10), 10 pg FoxD4/5 mRNA plus 10 pg Gro/Grg4 mRNA (Fox-10+Grg4-10), 100 pg wt-FoxD4/5 mRNA plus 20 ng GroMO (Fox-100+GroMO), 50 pg wt-FoxD4/5 mRNA (Fox-50), or 50 pg wt-FoxD4/5 mRNA plus 40 ng GroMO (Fox-50+2X GroMO). A low dose (10 pg) of either FoxD4/5 or Gro/Grg4 down-regulates the expression of these three genes in many fewer embryos than a higher dose of FoxD4/5 alone (100 pg). However, low doses of FoxD4/5 plus Gro/Grgr4 act synergistically to restore down-regulation at a level significantly higher than either mRNA alone (*, p<0.001), and at a level approaching 100 pg FoxD4/5 alone. However, MO knock-down of endogenous Gro/Grg4 expression does not reduce the ability of FoxD4/5 to cause down-regulation. At either a high (100 pg, black bar) or lower (50 pg, dark purple bar) dose of FoxD4/5, addition of GroMO did not significantly change the frequency of down-regulation of these genes (cf. to black bar to grey bar and dark purple bar to light purple bar). Numbers above bars indicate sample sizes.