Table 2.
Primer pairs and product sizes for selected gene transcripts studied in human retinal endothelial cells.
| Gene Transcript | Primer Pair (Product Size)Reference (if applicable) |
|---|---|
| Supervillin | for: 5′-TGGTGTTTGATTTTGGTAGTGAA-3′ rev: 5′-TAAGCGGATTGCATTCTCCA-3′(165 bp) |
| ICAM-1 | for: 5′-TAAGCCAAGAGGAAGGAGCA-3′ rev: 5′-CATATCATCAAGGGTTGGGG-3′ (282 bp)1 |
| VCAM-1 | for: 5′-CGTCTCATTGACTTGCAGCACC-3′ rev: 5′-GTGATCGGCTTCCCAGCCTC-3′ (276 bp) 2 |
| E-selectin | for: 5′-GAGCCTTCAGTGTACCTCATC-3′ rev: 5′-GACAATTCATGTAGCCTCGCTC-3′ (296 bp) 2 |
| P-selectin | for: 5′-GGATTGTTCTGACACTCGTGG-3′ rev: 5′-GAGGTTGGAGCAGTTCATCG-3′ (412 bp) |
| CD44 | for: 5′-ACATCAGTCACAGACCTGCC-3′ rev: 3′-GCAAACTGCAAGAATCAAAGCC-3′ (471 bp)3 |
| CXCL10 | for: 5′-AAGAGATGTCTGAATCCAGAATCGAAGG-3′ rev: 5′-CCTCAGTAGAGCTTACATTATAGTGCCAG-3′ (333 bp) |
| CCL20 | for: 5′-TGCTGTACCAAGAGTTTGCTC-3′ rev: 5′-GATTTGCGCACACAGACAAC-3′ (226 bp) |
| VEGF-165 | for: 5′-ATCTTCAAGCCATCCTGTGTGC-3′ rev: 5′-CAAGGCCCACAGGGATTTTC-3′ (224 bp)4 |
| GAPDH | for: 5′-AGCTGAACGGGAAGCTCACTGG-3′ rev: 5′-GGAGTGGGTGTCGCTGTTGAAGTC-3′ (209 bp)5 |
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