Figure 5. Significantly increased number of PD-1^hi gB-Tet⁻ CD8⁺ T cells in B7-H1^−/− TG.

TG from wild type or B7- H1^−/− C57BL/6 mice were obtained at 30 dpi (A, B, D, E) or at indicated times after infection (C), and dispersed cells were stained for CD45, CD8, gB_(498–505)-tetramer (gB-Tet), and PD-1, and analyzed by flow cytometry. A, bars represent the mean ± SEM number of gB-Tet⁺ and gB-Tet⁻ CD8⁺ T cells in TG of WT and B7-H1^−/− mice (n = 10 mice). B, Bars represent the mean ± SEM percentage of gB-Tet⁻ CD8⁺ T cells (n = 10 mice). C, Line graph showing the mean ± SEM percentage of gB-Tet⁺ CD8⁺ T cells at indicated time points in TG of WT and B7-H1^−/− mice (n = 5 mice). D, Representative dot plots showing PD-1 staining on gB-Tet⁺ and gB-Tet⁻ CD8⁺ T cells in TG of Wild type and B7H1^−/− mice. Bars indicate the mean ± SEM percentage of PD-1⁺ gB-Tet⁺ and gB-Tet⁻ CD8 T cells from WT and B7-H1^−/− mice (n= 10 mice). E, Bar graph showing PD-1 MFI for the gB-Tet⁺ and gB-Tet⁻ CD8⁺ T cells in wild type and B7-H1^−/− TG (n = 10 mice). All data are pooled from three independent experiments. *p ≤0.05, **p ≤ 0.01, and ***p ≤ 0.001