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. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Cell Metab. 2013 May 23;17(6):1000–1008. doi: 10.1016/j.cmet.2013.04.013

Figure 3. HnRNPA1-dependent splicing of the Myc-heterodimerization partner Max results in the truncated variant Delta Max.

Figure 3

(A) RT-PCR for Max intron upstream of exon 5 using RNA extracted from hnRNPA1 immunoprecipitated from U87-EGFRvIII cells after UV crosslinking (CLIP) as described in Supplemental Experimental Procedures.

(B) RT-PCR splicing analysis for Max exon 5 and immunoblot analysis for hnRNPA1 in neurospheres established from patient GBM biopsies.

(C) RT-PCR splicing analysis for Max exon 5 in hnRNPA1 knockdown cells from Figure 2E.

(D) Max immunoblot of lysates from U87-EGFRvIII cells transfected with siRNAs to hnRNPA1.

(E) Immunoblot for Max in Patient GBM biopsies from Figure 2C.