(A) RT-PCR for Max intron upstream of exon 5 using RNA extracted from hnRNPA1 immunoprecipitated from U87-EGFRvIII cells after UV crosslinking (CLIP) as described in Supplemental Experimental Procedures.
(B) RT-PCR splicing analysis for Max exon 5 and immunoblot analysis for hnRNPA1 in neurospheres established from patient GBM biopsies.
(C) RT-PCR splicing analysis for Max exon 5 in hnRNPA1 knockdown cells from Figure 2E.
(D) Max immunoblot of lysates from U87-EGFRvIII cells transfected with siRNAs to hnRNPA1.
(E) Immunoblot for Max in Patient GBM biopsies from Figure 2C.