. Author manuscript; available in PMC: 2014 Aug 1.

Published in final edited form as: Biochim Biophys Acta. 2013 Apr 11;1832(8):1279–1293. doi: 10.1016/j.bbadis.2013.04.004

Table 3.

Steady state kinetic constants for the reverse reaction of recombinant wild-type and active variant hGALT enzymes.

hGALT Variant	Apparent K_m or K_0.5 μM		Glc-1P K_i mM	UDP-Gal h	Apparent k_cat s⁻¹	Apparent k_cat/K_m or k_cat/K_0.5 × 10⁵ l.mol⁻¹.s⁻¹
hGALT Variant	UDP-Gal^a	Glc-1P^b				UDP-Gal^a	Glc-1P^b
WT	141 ± 5.2	159 ± 17	4.3 ± 0.9	2.9 ± 0.3	17.7 ± 0.4	1.26 ± 0.05	1.11 ± 0.12
p.D28Y	179 ± 12	121 ± 24.9	2.3 ± 0.6	1.4 ± 0.1	9.3 ± 0.3	0.52 ± 0.03	0.77 ± 0.16
p.F194L	89.7 ± 5.7	118 ± 14.4	3.2 ± 0.7	1.8 ± 0.2	5.5 ± 0.1	0.61 ± 0.04	0.47 ± 0.06
p.R333G	324.4 ± 33.6	202.1 ± 17.9	5.9 ± 1.2	1.6 ± 0.2	5.5 ± 0.3	0.16 ± 0.02	0.27 ± 0.03

[Glc-1P] = 1000 μM

[UDP-Gal] = 500 μM