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. Author manuscript; available in PMC: 2014 Aug 1.

Published in final edited form as: Biomaterials. 2013 May 25;34(26):6229–6238. doi: 10.1016/j.biomaterials.2013.04.061

Representative immunohistochemistry stain for micro vessel formation in the infarct border zone. Hearts were harvested immediately after the MRI analysis, and the isolated hearts were perfused, fixed, and sectioned through short axis. Immunohistochemical staining for (a) SM α-actin showing presence of arterioles and (b) CD31 detecting capillaries. (c) Average number of SMA positive arterioles and (d) average number of CD31 positive capillaries. The number of vessels in a 1 mm² area was recorded from five random high power fields in the infarct border zone per animal. Data presented as the mean + SD (**p<0.01).

Representative immunohistochemistry stain for micro vessel formation in the infarct border zone. Hearts were harvested immediately after the MRI analysis, and the isolated hearts were perfused, fixed, and sectioned through short axis. Immunohistochemical staining for (a) SM α-actin showing presence of arterioles and (b) CD31 detecting capillaries. (c) Average number of SMA positive arterioles and (d) average number of CD31 positive capillaries. The number of vessels in a 1 mm² area was recorded from five random high power fields in the infarct border zone per animal. Data presented as the mean + SD (**p<0.01).