Table 2. Donor and pancreas variables for human islet isolation data.
Variables |
no DI Serva (n=3) |
DI Serva (n=8) |
DI MTF (n=7) |
Age (years) |
41.3 ± 9.5 |
35.9 ± 3.9 |
43.0 ± 3.7 |
Gender (F/M) |
0/3 |
2/6 |
4/3 |
Body mass index (kg/m2) |
26.3 ± 3.1 |
32.2 ± 2.3 |
28.7 ± 1.2 |
Cold ischemic time (min) |
299 ± 60 |
172 ± 24 |
199 ± 11 |
Pancreas weight (g) |
104.4 ± 6.7 |
97.6 ± 10.1 |
101.7 ± 9.2 |
Phase I (min) |
17.3 ± 1.4 |
12.9 ± 1.1 |
13.0 ± 1.0 |
Phase II (min) |
52.7 ± 2.2 |
52.9 ± 3.7 |
47.6 ± 4.6 |
Undigested tissue (g) |
27.7 ± 7.7* |
14.9 ± 3.4 |
5.6 ± 0.9* |
Embedded islet rate (%) |
40.0 ± 20.5 |
31.4 ± 7.4 |
28.1 ± 4.1 |
Post-purification Purity (%) |
48.2 ± 10.3 |
56.6 ± 5.7 |
60.7 ± 3.0 |
Post-purification Viability (%) |
98.5 ± 0.2 |
97.8 ± 0.5 |
96.2 ± 0.5 |
Recovery rate (%) |
81.3 ± 12.9 |
74.5 ± 9.8 |
76.1 ± 6.8 |
Final pellet volume (ml) |
18.6 ± 6.5 |
8.2 ± 0.9 |
10.6 ± 3.3 |
Stimulation Index | 1.1 ± 0.2 | 7.8 ± 2.8 | 13.7 ± 2.9 |
Data are expressed as mean ± SE.* P < 0.05.