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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: J Immunol. 2013 May 17;190(12):6004–6014. doi: 10.4049/jimmunol.1203552

Figure 7. Blockade of PD-L1 on lamina propria APCs nullifies T cell tolerance.

Figure 7

LP APCs were harvested from C57BL/6 mice recipient of agg Ig-W + STI or agg Ig-MOG + STI, incubated with blocking anti-PD-L1 antibody and tested for tolerogenic function. (A–C) shows proliferation (A) and IFNγ (B) and IL-17 (C) production by 2D2 T cells incubated with MOG peptide-loaded LP APCs that were previously blocked with anti-PD-L1 antibody or isotype matched control. *p < 0.05. (D) shows the mean clinical score of EAE induced by MOG peptide in MHCII−/ − mice recipient of 2D2 T cells and LP APCs that were previously blocked with anti-PD-L1 antibody or isotype matched control. Data is representative of three independent experiments with 5 mice per group.