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Effect of tetrodotoxin (TTX) and proparacaine on cell viability in-vitro. Viability of (A) human corneal limbal epithelial cells and (B) corneal keratocytes was determined using the MTS assay. Data are means ± SD, n = 3. Comparisons at each time point were performed with the two-way ANOVA with Bonferroni post hoc test (* = p < 0.05). Citrate buffer 0.1 M, pH 4.5 was also studied, as it was the buffer in which tetrodotoxin was dissolved.
