Figure 3. Expression of KLRG1, CD27, and Sca-1 is differentially regulated on Ly49H⁺ and Ly49H⁻ NK cells during MCMV infection.

A) Representative dot plots showing KLRG1 (top), CD27 (middle), or Sca-1 (bottom) expression with respect to Ly49H expression and B) histograms showing the expression of these markers on Ly49H⁺ (solid line, no fill) or Ly49H⁻ (dashed line, grey fill) splenic NK cells from B6 mice 8 days p.i. MCMV. C) Proportion of Ly49H⁺ (♦, solid line) or Ly49H⁻ (◊, dashed line) splenic NK cells that express KLRG1, CD27, or Sca-1 from B6 mice 0, 2, 4, 6, 8, or 10 days p.i. MCMV. D) Frequency of CD27/Sca-1 subsets among Ly49H⁻KLRG1⁻ or Ly49H⁺KLRG1⁺ NK cells from wt B6 mice 4, 6 or 8 days p.i. MCMV. Sca-1⁺CD27⁻ (black), Sca-1⁺CD27⁺ (black stripes), Sca-1⁻CD27⁺ (black dots), Sca-1⁻CD27⁻ (no fill). E) Representative histogram showing frequency of Ly49H⁺ NK cells among NK cell subsets based on KLRG1, CD27, and Sca-1 coexpression patterns from wt B6 mice 8 days p.i. MCMV, which is quantified on the graph to the right as the ratio of Ly49H⁺ to Ly49H⁻ NK cells in each subset. All panels show cumulative results from 2, independent experiments with 4–5 mice/group/time point. Statistical significance was determined using paired, two-tailed t-tests. ***p < 0.001, **p < 0.01