Table 2B.
Brain amino acids levels in 1-year-old untreated Arg1−/−, AAV-mArg1-injected Arg1−/−, and littermate control mice. Values are given as mean ± SD (as nanomoles/mL per 100 mg of tissue). GB = γ-aminobutyric acid.
| Monoamino, Monocarboxylic | Branched Chain | Mercapto | Heterocyclic | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 Year | Alanine | Glycine | Isoleucine | Leucine | Valine | Cystathionine | Cystine | Taurine | Proline |
| Control Littermates | 92.1 ± 13.0 | 88.7 ± 10.8 | 4.7 ± 0.6 | 10.3 ± 1.2 | 11.9 ± 1.2 | 1.9 ± 0.4 | 0.36 ± 0.05 | 915.1 ± 81.7 | 8.4 ± 1.4 |
| Treated ARG1−/− | 100.1 ± 11.2 | 86.1 ± 9.9 | 5.3 ± 1.0 | 12.1 ± 1.4 | 11.6 ± 1.1 | 2.3 ± 1.0 | 0.22 ± 0.4 | 437.1 ± 76.7 | 9.4 ± 1.0 |
| p-value | 0.38 | 0.73 | 0.26 | 0.10 | 0.69 | 0.50 | 0.08 | 2.7E-05 | 0.27 |
| Diamino, Monocarboxylic | Monoamino, Dicarboxylic | Hydroxy | Brain-Related | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 Year | Histidine | Lysine | Glutamic acid | Aspartic acid | Serine | Threonine | Homocarnosine | Carnosine | GABA |
| Control Littermates | 9.6 ± 0.6 | 17.2 ± 1.4 | 1001.1 ± 80.8 | 368.5 ± 22.0 | 78.4 ± 12.9 | 32.4 ± 2.9 | 4.2 ± 1.0 | 14.5 ± 8.2 | 370.4 ± 62.7 |
| Treated ARG1−/− | 21.3 ± 4.4 | 17.4 ± 1.5 | 969.2 ± 96.3 | 319.7 ± 43.6 | 76.9 ± 11.7 | 13.3 ± 2.7 | 2.4 ± 0.9 | 12.6 ± 4.4 | 310.0 ± 45.9 |
| p-value | 0.0008 | 0.87 | 0.62 | 0.08 | 0.86 | 1.0E-05 | 0.03 | 0.69 | 0.16 |
| Aromatic | Urea Cycle Related | Carboxamide | Thioether | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 Year | Phenylalanine | Tyrosine | Tryptophan | Arginine | Citrulline | Ornithine | Asparagine | Glutamine | Methionine |
| Control Littermates | 7.7 ± 0.6 | 9.8 ± 1.1 | 2.9 ± 0.4 | 16.7 ± 1.8 | 2.0 ± 0.3 | 0.48 ± 0.1 | 9.8 ± 0.4 | 424.3 ± 48.2 | 5.6 ± 0.5 |
| Treated ARG1−/− | 10.2 ± 1.3 | 12.8 ± 1.6 | 4.3 ± 1.2 | 13.5 ± 1.3 | 2.9 ± 0.6 | 1.5 ± 0.3 | 10.4 ± 0.8 | 1304.8 ± 104.3 | 6.7 ± 0.4 |
| p-value | 0.009 | 0.01 | 0.07 | 0.03 | 0.03 | 0.0003 | 0.20 | 3.3E-07 | 0.008 |