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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Neurooncol. 2013 Mar 25;113(2):195–205. doi: 10.1007/s11060-013-1112-8

Fig. 4. mt-TTP has enhanced antiproliferative effects in glioma cells.

Fig. 4

A) Cell viability was determined after doxycycline induction of wt-TTP or mt-TTP by Trypan Blue exclusion counting (at baseline or after TNF-α stimulation). Data points are expressed as a percentage of uninduced cells and are the mean ± SD of three independent experiments. ***P <0.0005 comparing mt-TTP to wt-TTP cells. B) mt-TTP significantly reduces colony formation in soft agar compared to wt-TTP. Colonies were allowed to grow for 3 weeks and then counted. Data points represent the mean ± SD of three independent experiments. **P < 0.005, and *** P < 0.001 compared to Tet-On control cells; #P < 0.002. C) mt-TTP enhances chemosensitivity of glioma cells. Cells were induced with 0.25 μg/mL of doxycycline and treated with varying doses of temozolomide for 24 h and then assessed by an ATP-based luminescence assay. Data points are expressed as a percentage of untreated, doxycycline-induced cells (dose 0) and represent the mean ± SEM of at least 4 independent experiments. *P < 0.02; ** P < 0.008; **** P < 0.0001 comparing wt-TTP or mt-TTP to Tet-On control cells; #P < 0.01.