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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: J Immunol. 2013 May 13;190(12):6434–6449. doi: 10.4049/jimmunol.1202329

Fig. 4. Leukocyte-derived Adam8 predominantly mediates Adam8’s anti-inflammatory activities in the airways during AAI.

Fig. 4

We generated BALB/c strain Adam8 bone marrow (BM) chimeric mice, as described in Methods. Adam8 BM chimeric mice were sham-sensitized with PBS or sensitized with a low dose (10 μg) of OVA along with alum by the i.p. route and then challenged with aerosolized PBS or OVA. Lungs were inflated and fixed in formalin or BAL was performed. A shows images of hematoxylin and eosin-stained sections of lungs from the mice 24 h after the last OVA challenge (images are representative of 6 mice/group; original magnification × 100). PBS-treated mice had no airway inflammation (data not shown). Twenty four hours after the last OVA or PBS challenge, total leukocytes (B) and granulocytes (>80% eosinophils in C) were counted in BAL samples. Data are mean ± SEM n = 3–4 mice/group for PBS-treated mice and n = 8–22 mice/group for OVA-treated mice. Asterisk indicates p ≤ 0.01 and ** p ≤ 0.023 in both B and C.