Table 1.

Airway eosinophils and macrophages isolated from WT mice with AAI express Adam8 protein

	Adam8 protein levels (pg/μg total protein)**
BAL macrophages*	236.0 ± 65.7***
BAL eosinophils*	174.5 ± 24.2

^*BALB/c WT mice were sensitized with 10 μg of OVA by the i.p. route and then challenged with aerosolized OVA. Twenty four h after the last OVA challenge, airway eosinophils and macrophages were isolated, and cell extracts were prepared, as described in Materials and Methods.

^**Adam8 protein and total protein levels were measured in extracts of BAL macrophages versus BAL eosinophils using an ELISA and a Bradford dye-binding assay kit, respectively. The results are expressed as pg of Adam8 per μg of total protein.

^***Data are mean ± SEM; n = 16 different paired BAL macrophage and eosinophil preparations (p = 0.802 for the comparison of airway macrophages and eosinophils).