Skip to main content
. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: J Immunol. 2013 May 20;190(12):5921–5925. doi: 10.4049/jimmunol.1300847

FIGURE 4. C3aR/C5aR signaling modulates generation and stability of human iTreg.

FIGURE 4

(A) Percentage of Foxp3+ CD4+ cells on d5 of human iTreg induction cultures ± C3aR-A or C5aR-A (n=8). *p<0.05. (B) Suppressive capacities of CD25+ iTreg generated with C3aR-A (red), C5aR-A (blue) or PBS control (black). (C) Representative flow plot of DAF expression on humans DCs transfected with scrambled-targeted (black) or DAF-targeted (red) siRNA. (D) Plots depicting human Foxp3+ CD4+ cells generated with control or DAFkd DCs, ± C3aR-A (mean % Foxp3+ cells shown, n=4,*p<0.05 vs. other 2 groups). (E) Percentages and (F) total numbers of Foxp3 expressing, CD25-enriched human iTreg restimulated for 3d ± C3aR-A or C5aR-A. Numbers represent means and s.d. of 3 replicates, *p<0.05 vs. control. (G) Weights of NOD scid γcnull mice transferred with human PBMC treated with PBS (black), C5aR-A (red), CTLA4-Ig (blue), or CTLA-Ig + C5aR-A (green), n=4–6 per group *p<0.05 (CTLA4Ig vs. CTLA4Ig+C5aR-A), **p<0.05 vs. PBS and vs. C5aR-A alone. (H) Total numbers of human CD4+ cells (left) or CD4+Foxp3+ cells (middle) in the spleens, and percentages of Foxp3+ cells (right) within the human CD4+ gate, 6 wk after adoptive transfer (n=3–6/group), *p<0.05.