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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: J Immunol. 2013 May 15;190(12):5939–5948. doi: 10.4049/jimmunol.1203455

FIGURE 3. Siglec-F ligand expression on mouse lung cells is upregulated by intranasal administration of IL-4, IL-13, and Alternaria alternata in STAT6-dependent manner.

FIGURE 3

A, B. WT mice were intranasally challenged with control PBS, IL-4, or IL-13, and 24 hours later, their lungs were digested and analyzed for Siglec-F ligand expression. Expression on (A) epithelial cells and (B) indicated cell types from mouse lungs is shown. Shaded area show the staining with control human IgG, black lines show the staining on cells from mice with control PBS administration, and red lines show the staining on cells from mice with indicated cytokine challenges. Data are representatives from three separate experiments showing similar results. C, D. Both WT (C) and STAT6−/− (D) mice were intranasally administered Alternaria alternata extract 100 μg/mouse and 24 hours later, their airway epithelial cells were analyzed for Siglec-F ligand expression. Shaded area show the staining with control human IgG, black lines show the staining on cells from mice with control PBS administration, and red lines show the staining on airway epithelial cells from mice with A. alternata challenge. Data are representatives from two separate experiments showing similar results.