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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: Dev Biol. 2013 Apr 4;379(2):139–151. doi: 10.1016/j.ydbio.2013.03.019

Figure 7. Laminin is required to prevent SGP over-extension past the PGCs.

Figure 7

Stills from a 4D confocal movie of embryo expressing hnd-1P::GFP-PHPLC1∂1 (SGPs), end-1P::mCherry-PHPLC1∂1 (endoderm), and nmy-2P::PGL-1::RFP (PGC-specific P-granules). PGCs are indicated with asterisks. See Fig. 1G–J for comparison to wild type. In lam-1(RNAi) (A–B) and lam-2(RNAi) (D–E) embryos, SGPs extend projections around the PGCs (arrows), (C, F) but SGPs shift to lie posterior to the PGCs (white circles), and over-extend beyond the PGCs (vertical arrows indicate farthest posterior extensions). (G) Quantification of SGP over-extension beyond PGCs in live embryos of the indicated genotype at t=70′. To the right, a schematic of how measurements were taken in representative wild-type (top) and lam-1(RNAi) or lam-2(RNAi) embryos (bottom) is shown. Error bars are standard deviation. (*** = p<0.001, two-tailed Student’s t-test). n = 27, 20, and 43 embryos for control, lam-2(RNAi) and lam-1(RNAi) respectively. Scale bars are 5μm.