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. Author manuscript; available in PMC: 2014 Jun 6.
Published in final edited form as: Mol Cell. 2013 May 16;50(5):699–710. doi: 10.1016/j.molcel.2013.04.013

Fig. 2.

Fig. 2

Tip60 is essential for oncogenic ras-induced senescence.

(A) Semi-quantitative RT-PCR analysis of Tip60 mRNA levels in BJ cells transduced with GFP or indicated Tip60 shRNA using isoform-specific (D+C) or universal (Tot 5′+3′) primers (Fig. S2A–C).

(B) Western blot analysis of BJ cells transduced with GFP or indicated Tip60 shRNA.

(C) Population doubling of BJ cells transduced with GFP (shGFP) or Tip60 (shTip) shRNA and Ha-RasV12 or vector (WH).

(D) Population doubling of BJ cells transduced with GFP (shGFP) or Tip60 (shTIP887) shRNA, mouse Tip60 (mTip60) or vector (VT), and Ha-RasV12 or vector (WH). Insert, Western blotting of BJ cells transduced with GFP or Tip60 shRNA (shTip887) and FLAG-mTip60 or vector (VT).

(E) BJ cell populations in (C) and (D) were stained for SA-β-gal on day 18 post ras transduction.

(C–E) Values are mean ± SD for triplicates.

See also Figure S2.