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. Author manuscript; available in PMC: 2014 Jun 6.
Published in final edited form as: Mol Cell. 2013 May 16;50(5):699–710. doi: 10.1016/j.molcel.2013.04.013

Fig. 5.

Fig. 5

Tip60 acetylates PRAK in a phosphorylation dependent manner.

(A) His-PRAK was incubated with Tip60α, p38α, MKK6E, ATP and acetyl-CoA for an indicated period.

(B) His-PRAK was incubated with increasing amounts of Tip60α or buffer (−), p38α, MKK6E and acetyl-CoA with or without ATP.

(C) His-PRAK was incubated first with p38α, MKK6E and ATP, and then with wild type Tip60α (WT), Tip60α-A158 or buffer (−) and acetyl-CoA with or without SB203580.

(D) Wild type PRAK or PRAK-A182 was incubated with increasing amounts of Tip60α or buffer (−), p38α, MKK6E, ATP and acetyl-CoA.

(E) PRAK was incubated first with p38α and MKK6E with or without ATP, and then with Tip60α and acetyl-CoA with or without SB203580.

(A–E) Acetylation of PRAK and phosphorylation of PRAK and Tip60 were detected by Western blotting using anti-acetyl-Lys, anti-pPRAK-T182 and anti-pTip60-T158 antibodies, respectively. Input of recombinant proteins was stained by Ponceau.

See also Figure S5.