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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: Methods Mol Biol. 2010;629:141–158. doi: 10.1007/978-1-60761-657-3_10
48°C × 5 min
95°C × 10 min
95 °C × 30s
58°C × 30s for 40 cycles
95 °C × 15s
58 °C × 15s dissociation stage
95°C × 15s