Fig. 1.

Typical normal-phase LC-MS chromatograms of lipid extract from normolipidemic LDL in positive (+) (top) and negative (−) ion mode (bottom). LDL was extracted using solvent system 3 (acidified Bligh and Dyer method). Dashed boxes represent the elution time windows for lipid classes eluting (in order of appearance): 1, TAGs+CEs; 2, PCs; 3, SMs; 4, lysoPCs; 5, CS; 6, LAAs; 7, Cer+HexCer; 8, FAs+LacCer; 9, PIs; 10, PEs. Inset in the LC-MS chromatogram in negative ion mode depicts zoomed region for the elution of CS, LAAs, Cer and FAs.