TABLE 3.

Significance (P values) of the effect of solvent systems on the extraction of lipids calculated using data set 2 (all compounds in the elution zones)

Solvent Systems	Lipid Classes
	All	CSs	TAGs	CEs	FAs	LacCer	PIs	PEs	PCs	SMs	LysoPCs
All five	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	0.422	0.508	<0.001	<0.001
1 versus 2	<0.001	<0.001	<0.001	0.002	<0.001	0.01	<0.001	—	—	0.294	0.672
1 versus 3	0.008	0.004	0.002	<0.001	0.002	0.001	<0.001	—	—	<0.001	0.004
1 versus 4	0.008	0.598	0.002	0.002	0.012	0.001	0.004	—	—	<0.001	0.442
1 versus 5	0.002	0.004	0.006	<0.001	0.006	0.003	0.004	—	—	0.002	0.004
2 versus 3	0.062	0.632	0.034	0.082	0.528	0.001	0.02	—	—	0.002	<0.001
2 versus 4	0.002	0.004	0.006	<0.001	0.002	0.001	<0.001	—	—	<0.001	0.688
2 versus 5	0.002	0.004	0.004	0.004	0.002	0.002	<0.001	—	—	0.014	<0.001
3 versus 4	0.002	0.004	<0.001	<0.001	0.014	0.015	0.004	—	—	0.032	0.002
3 versus 5	0.006	0.002	0.002	0.002	0.01	<0.001	0.002	—	—	0.002	0.002
4 versus 5	0.002	0.006	<0.001	0.004	0.012	<0.001	0.044	—	—	0.002	0.004

P values were estimated using permutation test with 1,000 permutations. Bold text indicates a statistically significant effect (P < 0.05).