Fig. 6.

Silencing of PPARδ attenuates the stimulating effect of FAs on Plin gene transcription. C2C12 cells were transfected at day 0 with scramble or siRNA against PPARδ and differentiated for 2 days prior to stimulation for 24 h with BSA, GW501516 (0.1 μM), BSA-FAs (50 μM OA and 50 μM LA), or BSA-FAs in combination with antagonist to PPARδ (GSK0660; 1 μM). All wells received equal amounts of vehicle (0.1% DMSO) and BSA (40 μM). Relative expression of various RNAs against 36B4 was evaluated using quantitative real-time PCR analysis. Relative expression of Plin2, Plin4, Plin5, and PPARδ. Results are shown as mean ± SD (n = 3). Difference from control (BSA; *P > 0.05, **P > 0.01). Difference among scramble or PPARδ siRNA-treated cells receiving similar treatment (^#P > 0.01).