Fig. 8.

Oleic acid or PPARδ agonist treatment of mice elevates Plin5 in soleus muscle. Mice were gavaged twice (36 and 12 h before being euthanized) with vehicle (0.5% CMC), GW501516 (5 mg/kg), or 400 μl glyceryl trioleate/triolein. Mice were euthanized at the onset of the light cycle. A: Relative gene expression of Plin2-5 in soleus muscle relative to TBP. Results are presented as mean ± SEM (n = 4–6 per group). Statistical differences from CMC treatment (*P < 0.05, **P < 0.01). B: Western blot of perilipin4, perilipin5, and grp78 (control) proteins in soleus muscle. Each treatment is represented by four independent mice. C: Relative levels of perilipins/grp78 (n = 4, difference from CMC treatment, **P < 0.01).