Figure 3.

Stra6 expression is important for p53-mediated cell death. (a) qRT-PCR comparing the expression of Stra6 in HCT116 transfected with an empty vector (Control, Dox) or Stra6, treated or not with 1.4 μM doxorubicin for 24 h (Dox). (b) qRT-PCR measuring Stra6 mRNA levels in cells stably expressing shRNA against luciferase or Stra6, treated with 1.4 μM doxorubicin (Dox) for 24 h. Expression was normalized to that of TBP. Mean values and S.D. of three experiments are plotted. (c) Left column: Representative cell cycle profiles of HCT116 expressing shRNA against luciferase or Stra6, stained with PI (left column) or AnnexinV (right column). Cells were treated with 1.4 μM doxorubicin or 400 μM tBH for 48 h. Numbers indicate percentage of events in SubG₁ (left, dead cells) or Annexin V-positive cells (right, apoptotic cells). (d) Representative cell cycle profiles of PI-stained HCT116 (wt), HCT116 stably expressing shStra6 (shStra6), HCT116p53^−/− (p53^−/−) and HCT116Bax^−/− (Bax^−/−) treated with 1.4 μM doxorubicin for 48 h. Numbers indicate percentage of SubG₁ events (dead cells)