Figure 6.

R248Q/− lymphomas show increased in vivo proliferation compared with G245S/− and null lymphomas. Both mutant lymphomas show increased oncogenic signaling via the Akt pathway. (a, left) Immunohistochemical stainings for phospho-H3, TUNEL and p53 were performed on T lymphomas of null, R248Q/− and G245S/− tumors. (a, right) Quantitation of images shown on the left. Positive nuclei were counted in 10 random high-power fields ( × 40) per lymphoma. The mean of these averages for 10 (phospho-H3) and 5 (TUNEL) lymphomas per genotype are shown. Error bars indicate ±S.E. *P<0.05. (b) Primary normal thymocytes from 4-week-old p53+/+, p53−/− and p53 Q/− mice were compared with lysates from primary T lymphomas of the indicated genotypes. Immunoblot analysis for various signaling pathways. MAPK loading control. (c) Primary T-lymphoma lysates from G245S/− mice were compared with the first three T-lymphoma lysates from R248Q/− mice shown in (b). MAPK loading control