Figure 5.

Liver injury from GalN/TNF is increased in knockout (KO) mice. (a) Serum ALT levels in littermate control (Con) and KO mice 4 h after GalN/TNF treatment (*P<0.001 as compared with control mice; n=6-7). (b) TUNEL staining of control mouse liver 4 h after GalN/TNF. (c) TUNEL staining of 4 h GalN/LPS-treated KO liver. (d) Numbers of TUNEL-positive cells per high power field (HPF; *P<0.001 as compared with control mice; n=3-4). (e) Immunoblots of total hepatic protein from untreated and 4 h GalN/TNF-treated Con and KO mice for caspase-3 (Casp 3), caspase-7 (Casp 7), PARP and tubulin. Arrows indicate the procaspases (Pro), the cleaved caspase-3 (p17) and -7 (p30 and p19) forms, and the intact (p115) and cleaved (p85) forms of PARP