Figure 6.

CBP is the co-factor for c-Myc for Bim induction. (a and b) RNAi knockdown of CBP in MEFs results in lack of Bim induction up on βAR activation in western blot and in luciferase assays. (c) CBP-mediated Bim induction is dependent on intact c-Myc-binding sites on the Bim promoter. (d) HAT-deficient mutants of CBP fail to induce Bim. Acetylation of c-Myc by CBP or by the mutant variants in 293T cells is shown in the inset. (e) LK-2 cells that lack functional CBP respond to βAR signalling by inducing Bim protein in CBP-dependent manner. Error bars: ±S.E.M., n=3, #P=0.0022 versus untreated control (b); #P=0.008 versus isoproterenol-treated CBP control (c); #P=0.064 versus CBP control (e); paired one-tailed T-tests